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ATCC
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Image Search Results
Journal: bioRxiv
Article Title: Reticular adhesions: A new class of adhesion complex that mediates cell-matrix attachment during mitosis
doi: 10.1101/234237
Figure Lengend Snippet: (A,B) Gene-ontology analysis of U2OS steady state adhesion proteins (DMSO treated; focal and reticular adhesions) showing terms from Biological Process (A) and KEGG pathway analysis (B) significantly enriched over whole cell proteome. (C-E) Confocal images of U2OS β5 V cells attached to vitronectin (VN)-coated surfaces and immuno-labeled against EPS15L1 (C), HIP1R (D), or WASL (E). (F) Confocal images of U2OS cells cultured on glass coverslips for 72 h then treated with 20 μM CytoD for 2 h and immuno-labeled against integrin β5 and DAB2 along with staining of F-actin.
Article Snippet: Primary antibodies used for immunofluorescence and/or immuno-blotting include: anti-integrin β5 (15F11; MAB2019Z) (Millipore); anti-integrin αVβ5 (P1F6) (Abcam); polyclonal (rabbit) anti-integrin β5 (ab15459) (Abcam); anti-integrin β5 (4708S) (Cell Signalling Technology); anti-integrin αv (LM142) (Merck Millipore); anti-pan-talin (1 and 2) (53.8) (BioRad); anti-talin 1 (TA205) (Santa Cruz); anti-talin 2 (68E7) (Abcam); anti-integrin αVβ3 (LM609) (Abcam); anti-integrin β3 (AP3) (Abcam); anti-integrin β1 (LM534) (Millipore); anti-vinculin (hVIN-1) (Sigma Aldrich); anti-vinculin (V9131) (Sigma-Aldrich); anti-intersectin 1 (HPA018007) (Atlas Antibodies, Sigma-Aldrich); anti-NUMB (2733) (Cell Signaling Technologies); anti-EPS15L1 (HPA055309) (Atlas Antibodies, Sigma-Aldrich); anti-HIP1 (HPA013606) (Atlas Antibodies, Sigma-Aldrich); anti-WASL (HPA005750) (Atlas Antibodies, Sigma-Aldrich);
Techniques: Labeling, Cell Culture, Staining